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the peptidisc a simple method for stabilizing membrane proteins in detergent-free solution


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the peptidisc a simple method for stabilizing membrane proteins in detergent-free solution

Megabodies expand the nanobody toolkit for protein ...- the peptidisc a simple method for stabilizing membrane proteins in detergent-free solution ,The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. eLife 7, e34085 (2018). Frauenfeld, J. et al. A saposin-lipoprotein nanoparticle system for membrane proteins. Nat. Methods 13, 345–351 (2016). ...The importance of the membrane for biophysical ...Nov 16, 2020·The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. eLife 7 , e34085 (2018). PubMed PubMed Central Google Scholar



A step-by-step method for the reconstitution of an ABC ...

Aug 31, 2012·The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. Carlson ML, Young JW, Zhao Z, Fabre L, Jun D, Li J, Li J, Dhupar HS, Wason I, Mills AT, Beatty JT, Klassen JS, Rouiller I, Duong F. Elife, 7, 15 Aug 2018

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Development of methods for study of membrane proteins in ...

To this end, we design the peptidisc, a simple method for the universal stabilization of membrane protein complexes in detergent-free solution. Analysis of 5 different membrane protein complexes reconstituted in peptidiscs demonstrate that the method maintains function and increases stability of incorporated membrane proteins.

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ORCID

Oct 01, 2021·The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. eLife 2018-08-15 | Journal article PMID: 30109849 PMC: PMC6093710 DOI: 10.7554/elife.34085 Show more detail. Source: Franck Duong Van Hoa Lipopolysaccharides promote binding and unfolding of the antibacterial colicin E3 rRNAse domain. ...

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The Peptidisc, a simple method for stabilizing membrane ...

Aug 15, 2018·Thank you for submitting your work entitled "The Peptidisc, a Simple Method for Stabilizing Membrane Proteins in Detergent-free Solution" for consideration by eLife. Your article has been evaluated by a Senior Editor and three reviewers, one of whom, Volker Dötsch (Reviewer #1), is a member of our Board of Reviewing Editors.

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Stabilizing membrane proteins - ScienceDirect

Aug 01, 2001·Abstract. Membrane proteins can be extremely stable in a bilayer environment, but are often unstable and rapidly lose activity after detergent solubilization. Poor stability can preclude the detailed characterization of many membrane proteins. One way to alleviate this problem is to find more stable mutants of a membrane protein of interest.

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The high-throughput production of membrane proteins ...

Membrane proteins, found at the junctions between the outside world and the inner workings of the cell, play important roles in human disease and are used as biosensors. More than half of all therapeutics directly affect membrane protein function while nanopores enable DNA sequencing. The structural and functional characterisation of membrane proteins is therefore crucial. …

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Beatty Lab - Publications | Microbiology & Immunology UBC

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. ... The protein environment of the bacteriopheophytin anion modulates charge separation and charge recombination in bacterial reaction centers. J Phys Chem B. 117:7179–7189.

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‪John William Young‬ - ‪Google Scholar‬

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution ML Carlson, JW Young, Z Zhao, L Fabre, D Jun, J …

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Cycloalkane-modified amphiphilic polymers provide direct ...

Nov 25, 2021·The peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. Elife 7 , 1–23 (2018). CAS Article Google Scholar

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New malonate-derived tetraglucoside (MTG) detergents for ...

Jun 19, 2020·Membrane proteins are widely studied in detergent micelles, a membrane-mimetic system formed byamphiphilic compounds. However, classical detergents have serious limitations in their utility, particularly for unstable proteins such as eukaryotic membrane proteins and membrane protein complexes and thus there is an unmet need for novel …

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Improving antibody drug development using ...

The peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. eLife, 7 (2018), Article e34085. View Record in Scopus Google Scholar. 33. V.M. Luna, et al. Generation of membrane proteins in polymer-based lipoparticles as …

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Cryo‐EM of ABC transporters: an ice‐cold solution to ...

Nov 06, 2020·After solubilization, the detergent can also be exchanged into alternatives, such as amphipols or nanodisks. Of note, co-purification of annular lipids is commonly encountered, even when the protein is treated with detergent. However, it is desirable to keep the environment as close to native as possible and avoid the usage of detergent.

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Franck Duong - UBC Professor - Faculty Member - Researcher ...

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. (2018) eLife, Lipopolysaccharides promote binding and unfolding of the antibacterial colicin E3 rRNAse domain. (2017) Biochimica et biophysica acta. Biomembranes,

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Lipopeptide detergents for membrane protein studies

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution ... and, thereby, keep them soluble in detergent free aqueous solutions. ... Existing techniques for ...

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A novel lipoprotein nanoparticle system for membrane proteins

Feb 25, 2016·A saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposIn proteins. A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, …

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Single-Particle Cryo-EM of Membrane Proteins | SpringerLink

Apr 21, 2021·Carlson ML, Young JW, Zhao Z et al (2018) The peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. elife 7.

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A/Prof Isabelle Rouiller - University of Melbourne

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution Michael Luke Carlson, John William Young, Zhiyu Zhao, Lucien Fabre, Daniel Jun, Jianing Li, Jun Li, Harveer Singh Dhupar, Irvin Wason, Allan T Mills, J Thomas Beatty, John S Klassen, Isabelle Rouiller, Franck Duong

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PeptiQuick, a One-Step Incorporation of Membrane Proteins ...

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player.

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Phase separation in the isolation and purification of ...

Phase separation is a simple, efficient, and cheap method to purify and concentrate detergent-solubilized membrane proteins. In spite of this, phase separation is not widely used or even known among membrane protein scientists, and ready-to-use protocols are available for only relatively few detergent/membrane protein combinations.

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The Peptidisc, a simple method for stabilizing membrane ...

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution eLife ( IF 8.140) Pub Date : Membrane proteins are difficult to work with due to their insolubility in aqueous solution and quite often their poor stability in detergent micelles.

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HIV-1 Envelope and MPER Antibody Structures ... - Cell Reports

Apr 28, 2020·The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution. eLife. 2018; 7: e34085. Crossref; PubMed; Scopus (49) Google Scholar). In the lipid bicelle approach, similar detergent removal leads to heterogeneously sized bicelles capped by lipid molecules and/or scaffold. Formation of bicelles versus nanodiscs was ...

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2018 - Selected Publications | Facility for Electron ...

Carlson ML, Young JW, Zhao Z, Fabre L, Jun D, Li J, Li J, Dhupar HS, Wason I, Mills AT, Beatty JT, Klassen JS, Rouiller I, Duong F. 2018 The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution.

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Pursuing structural characterization of membrane proteins ...

Classical methods for reconstitution of membrane proteins in detergent-free buffer require extensive optimization and multiple purification steps. The recently developed membrane mimetic system - peptidisc - uses a short amphipathic peptide for fast and effective capture of membrane proteins in a single step. We demonstrate a new method to reconstitute proteins into the …

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‪Michael Carlson‬ - ‪Google Scholar‬

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution ML Carlson, JW Young, Z Zhao, L Fabre, D Jun, J …

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